Raman Spectroscopy

tecRaman technology facilitates precise 24/7 spectroscopy in industrial environments. Due to the spectroscopy principle of the scattering based laser Raman Spectroscopy a qualitative and quantitative analysis of complex mixtures is enabled. Maintenance free measurement points can be created for a variety of processes, including petrochemical, chemical, pharmaceutical and bio processes.

Raman Analysis
For Raman Spectroscopy of solid and liquid samples, a wide range of highly stable Raman probes are available: tecRaman probes for laboratory and process share the same highly stable optical setup so that knowledge gained in the laboratory can be used in processes. In addition, immersion probes are available. The process integration options include multichannel operation and installations in explosive atmospheres.
As an OEM supplier with many years of experience, tec5 offers the possibility of configuring spectrophotometer components and accessories for Raman Spectroscopy individually for process applications and integrating them into existing processes.

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tecRaman probe process laboratory

Applications of our customers realized with tecRaman technology:

tec5 Raman spectroscopy for Bioprocess Monitoring of Cell Cultivations
The use of spectroscopy for bioprocess monitoring is a powerful tool within PAT. In-line measurements are particularly important during cost-intensive manufacturing of biopharmaceuticals in order to facilitate early process fault detection, minimize the risk of contamination and observe real time product release. In this initial study, the new non-contact tecRaman process probe by tec5 was used for monitoring of CHO cell cultivations for monoclonal antibody production in a stirred tank bioreactor. Please find all details on the poster below.
Monitoring of Biocatalytic Synthesis of Naproxen by Raman Spectroscopy

Biological transformations are increasingly used in the production of fine chemicals or pharmaceutical ingredients. Process monitoring following the synthesis of Naproxen in a biocatalytic synthesis was demonstrated within this poster and published in the following paper.
Non-contact Raman spectroscopy for in-line monitoring of glucose and ethanol during yeast fermentations
The monitoring of microbiological processes using Raman spectroscopy has gained in importance over the past few years. Commercial Raman spectroscopic equipment consists of a laser, spectrometer, and fiberoptic immersion probe in direct contact with the fermentation medium. To avoid possible sterilization problems and biofilm formation on the probe tip, a large-aperture Raman probe was developed. The design of the probe enables non-contact in-line measurements through glass vessels or inspection glasses of bioreactors and chemical reactors. The practical applicability of the probe was tested during yeast fermentations by monitoring the consumption of substrate glucose and the formation of ethanol as the product. Multiple linear regression models were applied to evaluate the Raman spectra. Reference values were determined by high-performance liquid chromatography. The relative errors of prediction for glucose and ethanol were 5 and 3%, respectively. The presented Raman probe allows simple adaption to a wide range of processes in the chemical, pharmaceutical, and biotechnological industries.
Non invasive process probe for inline Raman monitoring through optical inspection glasses

Raman spectroscopy features some significant advantages against existing spectroscopic techniques. Amongst them are higher chemical sensitivities compared to NIR and a relatively low detection range for water molecules, in contrast to MIR spectroscopy. In process installations, compared to all spectroscopic techniques Raman plays only a minor role. Main challenges for process integration are long-term stability of the available measurement systems, high installation costs of so-called immersion probes and the addition of measurement points to existing plant layouts in piping and vessels.Read the complete abstract in the following link.

Non-Invasive Raman Reaction-Monitoring in Microchannels 

In-line Raman spectroscopy is an efficient tool for investigating kinetics of chemical reactions or for monitoring analyte concentrations nearly in real time. Compared to off-line product analysis like HPLC or GC, no quenching or further sample preparation is required. Both posters describe the use of a MultiSpec® Raman system for kinetic studies.

Efficient Kinetic Measurements in Microreactors by in-line Raman Spectrometer

Monitoring of Kinetics in microreactors can be carried out by in-line Raman spectroscopy. Hereby, an efficient analysis of rapid and exothermal reactions can be achieved. Furthermore, a much faster and cost efficient analysis,  avoiding additional steps such as quenching, probe preparation and off-line analyses can be reached.

Process Raman Spectroscopy for the in-line Monitoring of aerob Fermentation 

Automated control of processes for the chemical and biotechnological industry requires measurement technologies, which directly [in-line or on-line] determine the reactands and products concentration. Furthermore, a direct and easy integration in the process plant is required. This poster describes the monitoring of a fermentation with an in-line Raman spectrometer.

Process Capable Raman Spectroscopy with High Optical Efficiency for in-line Analytics 

Established off-line methods for industrial monitoring of chemical reactions is increasingly replaced by optical online methods. A research project at University of Applied Sciences Mannheim developed a high efficient Raman setup based on 532nm laser excitation and tec5 operation electronics for S7031-1006S.