Concentration Measurement by Optical Spectroscopy

Optical spectroscopic techniques, such as UV-VIS-NIR and Raman, allow for the atline and inline, real-time analysis of multiple parameters for quality control. These analytical techniques have been used for concentration measurements in various applications and are easily adapted to different sampling methods including fiber-optic immersion probes, reflection probes and flow-through cells. In UV-VIS-NIR spectroscopy, the sample is illuminated by a continuous, white light source, and the transmitted or reflected light measured, allowing the determination of the absorbance of the light as a function of wavelength. The absorption depends on the electronic and atomic structure of the absorbing molecule. The Lambert-Beer law relates the absorption of light to the concentration of the absorbing species.
In Raman spectroscopy, the sample is illuminated using a single-wavelength laser source. The energy of the inelastic scatter, Raman scattering, differs from the input wavelength by discrete amounts, which depends on the vibrational frequencies of the sample. The Raman signal directly correlates with the concentration of the measured molecule.

Learn more about suitable products …

8-Channel HPLC Detector

The customer is a specialized company in the instrumental analytic business. Sepiatec develops separation systems based on HPLC and SFC technology, enabling their customers to speed up their separation and method development. The systems of Sepiatec are used around the world by major pharmaceutical and biotechnology companies as well as by research institutes.

The new HPLC detector design is capable of measuring 8 channels in parallel, using the tec5 electronic multiplexer MUX-8A in combination with the Carl Zeiss MMS monolithic spectrometers, which proved suitable for the application. The first generation of instruments was equipped with a mechanical fiber-optic multiplexer. All measurements were done sequentially. 
The parallel operation of 8 MMS spectrometers now optimizes speed and signal-to-noise ratio significantly, which brings Sepiatec a big advantage in the market.